Vinculin Monoklonaler Antikörper

Vinculin Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A11F8

Kat-Nr. : 66305-2-PBS

Synonyme

3A11F8, CMD1W, Metavinculin, MV, MVCL

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  WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 66305-2-Ig (same clone as 66305-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 66305-2-Ig (same clone as 66305-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 66305-2-Ig (same clone as 66305-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody (66305-2-Ig, Clone: 3A11F8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66305-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Vinculin und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Vinculin fusion protein Ag24946
• Vollständiger Name: vinculin
• Berechnetes Molekulargewicht: 1133 aa, 124 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC039174
• Gene symbol: Vinculin
• Gene ID (NCBI): 7414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.