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Vinculin Polyklonaler Antikörper

Vinculin Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

Kat-Nr. : CL594-26520

Synonyme

VCL, MVCL, MV, Metavinculin, CMD1W

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite®594-conjugated Vinculin Polyclonal antibo (CL594-26520)

    IF Staining of HUVEC using CL594-26520

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated Vinculin Polyclonal antibo (CL594-26520)

    IF Staining of A549 using CL594-26520

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:200, CL488-phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Vinculin Polyclonal antibo (CL594-26520)

    IF Staining of HepG2 using CL594-26520

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:200, CL488-phalloidin (green).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHepG2-Zellen, A549-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

CL594-26520 bindet in WB, IF/ICC Vinculin und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Vinculin fusion protein Ag24942
Vollständiger Name vinculin
Berechnetes Molekulargewicht 1133 aa, 124 kDa
Beobachtetes Molekulargewicht 117 kDa
GenBank-ZugangsnummerBC039174
Gene symbol Vinculin
Gene ID (NCBI) 7414
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL594 Vinculin antibody CL594-26520Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Front Pharmacol

Polydatin enhances oxaliplatin-induced cell death by activating NOX5-ROS-mediated DNA damage and ER stress in colon cancer cells

Authors - Qi Zhao
View Article