Vitamin D binding protein Monoklonaler Antikörper

Vitamin D binding protein Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1E4D10

Kat-Nr. : 66175-1-Ig

Synonyme

DBP, DBP/GC, GC, Gc globulin, VDB, VDBG, VDBP, VDBP,GC, Vitamin D binding protein

---

Galerie der Validierungsdaten

• 

  WB analysis of human testis using 66175-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U-937 using 66175-1-Ig

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in FC: U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

66175-1-Ig bindet in WB, IHC, IF, FC, CoIP, ChIP, ELISA Vitamin D binding protein und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Vitamin D binding protein fusion protein Ag9803
• Vollständiger Name: group-specific component (vitamin D binding protein)
• Berechnetes Molekulargewicht: 474 aa, 53 kDa
• Beobachtetes Molekulargewicht: 52-58 kDa
• GenBank-Zugangsnummer: BC057228
• Gene symbol: GC
• Gene ID (NCBI): 2638
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

Publikationen

Species	Application	Title
mouse	IHC,IF,WB,CoIP,ChIP	Theranostics; Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma; Authors - Lu-Ning Qin