Vitamin D binding protein Monoklonaler Antikörper

Vitamin D binding protein Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4D10

Kat-Nr. : CL488-66175

Synonyme

GC, VDBP,GC, Gc globulin, DBP-maf, DBP/GC

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  IF Staining of U-937 using CL488-66175

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells using CoraLite® Plus 488 Vitamin D binding protein antibody (CL488-66175, Clone: 1E4D10 ) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-66175

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Vitamin D binding protein antibody (CL488-66175, Clone: 1E4D10 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: U-937-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66175 bindet in IF/ICC Vitamin D binding protein und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Vitamin D binding protein fusion protein Ag9803
• Vollständiger Name: group-specific component (vitamin D binding protein)
• Berechnetes Molekulargewicht: 474 aa, 53 kDa
• Beobachtetes Molekulargewicht: 52-58 kDa
• GenBank-Zugangsnummer: BC057228
• Gene symbol: Vitamin D binding protein
• Gene ID (NCBI): 2638
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.