WAPL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WAPL Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Rezensionen (1)

Kat-Nr. : 16370-1-AP

Synonyme

WAPL/FOE, FOE, Wings apart-like protein homolog, WAPL cohesin release factor, WAPAL

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  WB analysis using 16370-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 16370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 16370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16370-1-AP (WAPL; WAPAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16370-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WAPL; WAPAL antibody (16370-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 2 publications below
• ChIP: See 3 publications below

Produktinformation

16370-1-AP bindet in WB, IHC, IF/ICC, ChIP, ELISA WAPL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WAPL fusion protein Ag9280
• Vollständiger Name: wings apart-like homolog (Drosophila)
• Berechnetes Molekulargewicht: 142 kDa
• Beobachtetes Molekulargewicht: 160-180 kDa
• GenBank-Zugangsnummer: BC017393
• Gene symbol: WAPL
• Gene ID (NCBI): 23063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,ChIP	Nat Genet; WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation.; Authors - Ning Qing Liu ; KO Validated
human	WB	Sci Adv; RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis.; Authors - Shu Zhang
mouse	WB	J Clin Invest; Allogeneic T cell responses are regulated by a specific miRNA-mRNA network.; Authors - Sun Yaping Y
mouse	WB	Genome Biol; CTCF and cohesin promote focal detachment of DNA from the nuclear lamina; Authors - Tom van Schaik
human	WB	Curr Biol; Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2.; Authors - Masashi Minamino
mouse	WB,IF	Cell Rep; Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.; Authors - Miguel A Brieño-Enríquez

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhenmin (Verified Customer) (12-05-2019)

This antibody is very specific and works very well with immunohistochemical staining for formalin fixed and paraffin embedded tissue.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Testis

WAPL Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse Testis (Cat no:16370-1-AP)