WASF3 Monoklonaler Antikörper

WASF3 Monoklonal Antikörper für IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B7D10

Kat-Nr. : 67620-1-PBS

Synonyme

Brush 1, KIAA0900, Protein WAVE 3, SCAR3, WAS protein family, member 3, WASF3, WASP family protein member 3, WAVE3

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  WB analysis using 67620-1-Ig (same clone as 67620-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67620-1-Ig (WASF3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67620-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67620-1-Ig (same clone as 67620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67620-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67620-1-Ig (same clone as 67620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67620-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67620-1-Ig (same clone as 67620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67620-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67620-1-Ig (same clone as 67620-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67620-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 67620-1-Ig (same clone as 67620-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67620-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67620-1-Ig (same clone as 67620-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67620-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67620-1-PBS bindet in IHC, IF-P, Indirect ELISA WASF3 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WASF3 fusion protein Ag29991
• Vollständiger Name: WAS protein family, member 3
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-55 and 66-70 kDa
• GenBank-Zugangsnummer: BC050283
• Gene symbol: WASF3
• Gene ID (NCBI): 10810
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WASF3, also named WAVE3 or SCAR3, belongs to the SCAR/WAVE family. It plays a role in the regulation of cell morphology and cytoskeletal organization. WASF3 is associated with movement, invasion, and metastasis in different cancers such as breast cancer, gastric cancer, and prostate cancer. It has been shown that WASF3 levels are elevated in advanced-stage cancers compared to lower-grade tumors and normal tissue. The 67620-1-Ig antibody recognizes 50-55 kDa and 66-70 kDa proteins similar to the papers.(PMID: 21544801, 15826941, 31038356, 27432794, 31542393)