WASL Polyklonaler Antikörper

WASL Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 14306-1-AP

Synonyme

Actin nucleation-promoting factor WASL, N WASP, Neural Wiskott-Aldrich syndrome protein, NWASP, N-WASP

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  WB analysis of mouse brain using 14306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14306-1-AP (WASL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14306-1-AP (WASL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14306-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14306-1-AP (WASL antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14306-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14306-1-AP (WASL antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14306-1-AP

  Immunofluorescent analysis of HepG2 cells, using WASL antibody 14306-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

14306-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA WASL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WASL fusion protein Ag5424
• Vollständiger Name: Wiskott-Aldrich syndrome-like
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC052955
• Gene symbol: WASL
• Gene ID (NCBI): 8976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Immunol; FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.; Authors - Chunlin Wang
mouse,human	WB,IF,CoIP	iScience; WASH interacts with Ku to regulate DNA double-stranded break repair.; Authors - Tao Wang
human	WB	J Dermatol Sci; Negative pressure accelerated monolayer keratinocyte healing involves Cdc42 mediated cell podia formation.; Authors - Hsu Chih-Chin CC
human	WB	Neuroreport; Decreased expression of cortactin in the schizophrenia brain.; Authors - Hriday P Bhambhvani
human	WB,IHC,IF	Noncoding RNA Res; ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma; Authors - Haoyuan Xu
human	IF	Mol Cell; The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.; Authors - Joanna R Kovalski