WBP2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

WBP2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2B4G7

Kat-Nr. : 66585-1-Ig

Synonyme

WBP 2, WBP2, WW domain binding protein 2

---

• 

  WB analysis of HEK-293 using 66585-1-Ig

  WB result of WBP2 antibody (66585-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WBP2 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 66585-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66585-1-Ig (WBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66585-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66585-1-Ig (WBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 66585-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66585-1-Ig (WBP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66585-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66585-1-Ig (WBP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66585-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WBP2 antibody (66585-1-Ig, Clone: 2B4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 66585-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WBP2 antibody (66585-1-Ig, Clone: 2B4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IF: See 1 publications below

Produktinformation

66585-1-Ig bindet in WB, IHC, IF/ICC, ELISA WBP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WBP2 fusion protein Ag2661
• Vollständiger Name: WW domain binding protein 2
• Berechnetes Molekulargewicht: 261 aa, 28 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC010616
• Gene symbol: WBP2
• Gene ID (NCBI): 23558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Cell Death Dis; WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.; Authors - Zhe Zheng ; KO Validated