WBP2NL Polyklonaler Antikörper

WBP2NL Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 22587-1-AP

Synonyme

PAWP, Postacrosomal sheath WW domain-binding protein, WW domain-binding protein 2-like

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  WB analysis using 22587-1-AP

  Human testis tissue were subjected to SDS PAGE followed by western blot with 22587-1-AP (WBP2NL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22587-1-AP

  Mouse testis tissue were subjected to SDS PAGE followed by western blot with 22587-1-AP (WBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat epididymis using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat epididymis tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat epididymis using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat epididymis tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 22587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22587-1-AP (WBP2NL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, Maus-Eierstockgewebe, Ratten-Eierstockgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 4 publications below

Produktinformation

22587-1-AP bindet in WB, IHC, IF, ELISA WBP2NL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WBP2NL fusion protein Ag18347
• Vollständiger Name: WBP2 N-terminal like
• Berechnetes Molekulargewicht: 309 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC022546
• Gene symbol: WBP2NL
• Gene ID (NCBI): 164684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WBP2NL, also known as PAWP, is a protein found in the post-acrosomal region of mammalian spermatozoa. It was initially proposed as a sperm-borne oocyte-activating factor (SOAF) that triggers calcium oscillations essential for oocyte activation and embryo development. However, recent studies have shown mixed results regarding its role in fertilization. While some investigations suggest that PAWP can induce oocyte activation, others indicate that it may not be sufficient to compensate for the absence of other critical factors like PLCZ1. Additionally, altered WBP2NL expression has been observed in breast cancer tissues, hinting at its potential involvement in oncogenic regulation. (PMID: 24907910, PMID: 25417742, PMID: 28663133)

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; CEP128 is involved in spermatogenesis in humans and mice.; Authors - Xueguang Zhang
human	WB	Hum Mol Genet; Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW binding protein PAWP.; Authors - Jessica Escoffier
	WB,IF	Int J Mol Sci; Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers.; Authors - Jaromír Vašíček
mouse	WB	Development; PLCζ is the physiological trigger of the Ca(2+) oscillations that induce embryogenesis in mammals but offspring can be conceived in its absence.; Authors - Alaa Hachem
human	WB,IF	Mol Hum Reprod; Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.; Authors - Michail Nomikos
human	WB	Mol Hum Reprod; Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm.; Authors - Junaid Kashir