WBSCR22 Polyklonaler Antikörper

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• KD/KO Validated

WBSCR22 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 28192-1-AP

Synonyme

Bud site selection protein 23 homolog, BUD23, HASJ4442, HUSSY 3, MERM1, Metastasis-related methyltransferase 1, PP3381, Probable 18S rRNA (guanine-N(7))-methyltransferase, rRNA methyltransferase and ribosome maturation factor, WBMT, WBSCR22

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  WB analysis using 28192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28192-1-AP (WBSCR22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28192-1-AP

  WB result of WBSCR22 antibody (28192-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WBSCR22 transfected HeLa cells.

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  IHC staining of human colon cancer using 28192-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28192-1-AP (WBSCR22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

28192-1-AP bindet in WB, IHC, IF, ELISA WBSCR22 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WBSCR22 fusion protein Ag28094
• Vollständiger Name: Williams Beuren syndrome chromosome region 22
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000169
• Gene symbol: WBSCR22
• Gene ID (NCBI): 114049
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WBSCR22 (Williams-Beuren syndrome chromosomal region 22 protein), also known as BUD23, MERM1, is affected in Williams-Beuren syndrome and has been implicated in tumorigenesis and metastasis formation. WBSCR22 contains a nuclear localization signal and a common S-adenosyl-L-methionine binding motif that is evolutionarily conserved in methyltransferases (PMID: 29133897, 25525153). WBSCR22 protein is expressed at a high level in invasive breast cancer and its ectopic expression enhances tumor cell survival in the vasculature (PMID: 24086612).

Publikationen

Species	Application	Title
human	IF,WB	Heliyon; RNA-related DNA damage and repair: The role of N7-methylguanosine in the cell nucleus exposed to UV light; Authors - Lenka Stixová
human	WB	J Transl Med; Histidine metabolism drives liver cancer progression via immune microenvironment modulation through metabolic reprogramming; Authors - Pengcheng Liu ; KD Validated