WDR18 Polyklonaler Antikörper

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• KD/KO Validated

WDR18 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15165-1-AP

Synonyme

R32184_1, WD repeat domain 18, WDR18

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 15165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15165-1-AP (WDR18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15165-1-AP (WDR18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15165-1-AP (WDR18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15165-1-AP (WDR18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15165-1-AP (WDR18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15165-1-AP (WDR18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15165-1-AP (WDR18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15165-1-AP (WDR18 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Lungenkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

15165-1-AP bindet in WB, IP, IHC, ELISA WDR18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR18 fusion protein Ag7282
• Vollständiger Name: WD repeat domain 18
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC001648
• Gene symbol: WDR18
• Gene ID (NCBI): 57418
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR18 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit. This complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation (PMID:21326211).

Publikationen

Species	Application	Title
human	WB	EMBO J; The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.; Authors - Finkbeiner Elisabeth E ; KD Validated
human	WB	Mol Cell Proteomics; Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.; Authors - Fanis Pavlos P
human	WB	PLoS One; A Role of hIPI3 in DNA Replication Licensing in Human Cells.; Authors - Yining Huang
human	IP,WB	Mol Oncol; PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis; Authors - Xue Yang