WDR21C Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

WDR21C Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240402H3

Kat-Nr. : 83802-4-RR

Synonyme

240402H3, DCAF4L2, DDB1- and CUL4-associated factor 4-like protein 2, WD repeat domain 21C, WD repeat-containing protein 21C

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  WB analysis using 83802-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83802-4-RR (WDR21C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83802-4-RR

  WB result of WDR21C antibody (83802-4-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR21C transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 83802-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83802-4-RR against Human WDR21C were performed. The affinity constant is 0.479 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83802-4-RR bindet in WB, ELISA WDR21C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: WDR21C fusion protein Ag30748
• Vollständiger Name: WD repeat domain 21C
• Berechnetes Molekulargewicht: 395 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC111778
• Gene symbol: WDR21C
• Gene ID (NCBI): 138009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR21C (WD repeat-containing protein 21C), also known as DCAF4L2. It is expected to be located in the cytoplasm, which is enriched in testis. WDR21C is a member of WD-repeat proteins, which commonly serve as mediators of protein-protein interplay. It is reported that elevated WDR21C expression in human colorectal cancer (CRC) significantly correlated with a more advanced clinical stage as in lymphatic and distant metastasis. More importantly, elevated WDR21C expression is an independent prognosis factor for survival (PMID: 27158335). The molecular weight of WDR21C is 43 kDa.