WDR24 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

WDR24 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251220G1

Kat-Nr. : 86464-1-RR

Synonyme

C16orf21, EC:2.3.2.27, GATOR2 complex protein WDR24, JFP7, WD repeat-containing protein 24

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  WB analysis using 86464-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86464-1-RR (WDR24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 86464-1-RR

  WB result of WDR24 antibody (86464-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 86464-1-RR

  IP result of anti-WDR24 (IP:86464-1-RR, 4ug; Detection:86464-1-RR 1:2000) with HEK-293 cells lysate 1200 ug.

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  Affinity and Kinetic Characterization of 86464-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86464-1-RR against Human WDR24 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, HuH-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86464-1-RR bindet in WB, ELISA WDR24 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: WDR24 fusion protein Ag14774
• Vollständiger Name: WD repeat domain 24
• Berechnetes Molekulargewicht: 920 aa, 102 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC008025
• Gene symbol: WDR24
• Gene ID (NCBI): 84219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)