WDR32 Polyklonaler Antikörper

WDR32 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20483-1-AP

Synonyme

DCAF10, WD repeat domain 32, WDR32

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  WB analysis of HeLa using 20483-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20483-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 20483-1-AP

  IP result of anti-WDR32 (IP:20483-1-AP, 4ug; Detection:20483-1-AP 1:300) with HepG2 cells lysate 6000ug.

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  IHC staining of human testis using 20483-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 20483-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 20483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20483-1-AP

  Immunofluorescent analysis of HepG2 cells using 20483-1-AP (WDR32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20483-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA WDR32 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR32 fusion protein Ag14360
• Vollständiger Name: WD repeat domain 32
• Berechnetes Molekulargewicht: 559 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC003520
• Gene symbol: WDR32
• Gene ID (NCBI): 79269
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR32, containing seven WD repeats, is also termed DDB1- and CUL4-associated factor 10 (DCAF10), which may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (PMID: 16949367). WDR32 is able to interact with DDB1 (PMID: 16949367). WDR32 is supposed to be implicated in process of ubiquitin-dependent protein degradation system. Two isoforms are described and produced by alternative splicing. Proteintech's WDR32 antibody 20483-1-AP majorly detects WDR32 isoform1 with a theoretical MW of 61 kDa.