WDR43 Polyklonaler Antikörper

WDR43 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30227-1-PBS

Synonyme

KIAA0007, WD repeat domain 43

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  WB analysis using 30227-1-AP (same clone as 30227-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30227-1-AP (WDR43 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 30227-1-AP (same clone as 30227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 30227-1-AP (same clone as 30227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 30227-1-AP (same clone as 30227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat ovary using 30227-1-AP (same clone as 30227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 30227-1-AP (WDR43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30227-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30227-1-PBS bindet in WB, IHC, Indirect ELISA WDR43 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR43 fusion protein Ag30585
• Vollständiger Name: WD repeat domain 43
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_015131
• Gene symbol: WDR43
• Gene ID (NCBI): 23160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR43, a WD40 domain-containing protein, is a conserved component of the small-subunit processome (SSUP), which mediates pre-18S ribosomal RNA (rRNA) transcription and processing in the nucleolus and is critical for ribosome biogenesis in yeast. Its mutation in zebrafish causes a variety of early developmental defects in specific tissues. WDR43 directly promotes ESC self-renewal by maintaining high-level expression of its target genes involved in pluripotency programs. (PMID: 28880863, 24497835, 31128943)