WDR45/WIPI4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WDR45/WIPI4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 19194-1-AP

Synonyme

JM5, WD repeat domain 45, WDR45, WDRX1, WDRXI4, WIPI 4, WIPI4

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 19194-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 19194-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19194-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 19194-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19194-1-AP (WDR45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 19194-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19194-1-AP (WDR45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IP: See 1 publications below

Produktinformation

19194-1-AP bindet in WB, IP, IHC, IF, ELISA WDR45/WIPI4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR45/WIPI4 fusion protein Ag6741
• Vollständiger Name: WD repeat domain 45
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC000464
• Gene symbol: WDR45
• Gene ID (NCBI): 11152
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).

Publikationen

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	WB,IP	Sci Adv; Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism.; Authors - Alexander S Häusl ; KD Validated
human	WB	Int J Mol Sci; Iron Accumulation and Changes in Cellular Organelles in WDR45 Mutant Fibroblasts.; Authors - Hye Eun Lee ; KD Validated
human	WB	J Neurochem; A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation; Authors - Luisa Aring ; KO Validated
human	WB	Am J Med Genet A; Autophagic defects observed in fibroblasts from a patient with β-propeller protein-associated neurodegeneration.; Authors - Jae-Hyeok Lee
human	WB	Eur J Med Genet; Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants.; Authors - Hongbo Chen