WDR5 Polyklonaler Antikörper

WDR5 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 15544-1-AP

Synonyme

BIG 3, BIG3, BMP2-induced 3-kb gene protein, CFAP89, SWD 3

---

• 

  WB analysis using 15544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15544-1-AP (WDR5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15544-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15544-1-AP (WDR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15544-1-AP (WDR5 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 15544-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using WDR5 antibody (15544-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

15544-1-AP bindet in WB, IHC, IF/ICC, CoIP, ChIP, ELISA WDR5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR5 fusion protein Ag7900
• Vollständiger Name: WD repeat domain 5
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC001635
• Gene symbol: WDR5
• Gene ID (NCBI): 11091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The WDR5 antibody targets the WD repeat protein domain 5 (WDR5), a member of the WD40 repeat protein family in humans. The WDR5 protein is a core member of the mammalian trithorax complex and, like the rest of the WD40 repeat protein family, is primarily involved in diverse cellular processes. WDR5 is identified as a methyl Lys-4 H3-specific-binding protein, however it is known to interact with the Mixed Lineage Leukemia (MLL) protein at the same site for histone H3 tri-methylation(PMID:18840606). Recently, the WDR5 protein has been shown to regulate embryonic stem cell self-renewal, and an increased expression of WDR5 has been found to efficiently induce the formation of pluripotent stem cells (PMID:21477851). WDR5 is also shown to accelerate osteoblast and chondrocyte differentiation(PMID:16730692 ).

Publikationen

Species	Application	Title
human	WB	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	WB	Sci Adv; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency; Authors - Chikako Okubo
human	ChIP	Sci Adv; A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression.; Authors - Austin G Holmes
human	WB,IF	Proc Natl Acad Sci U S A; Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development.; Authors - Ma Pengpeng P
human	WB	Pharmacol Res; HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5; Authors - Fu-Chun Huo
mouse	WB	Nat Aging; Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging; Authors - Meiyang Li