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WDR74 Rekombinanter Antikörper

WDR74 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251046A3

Kat-Nr. : 86361-2-PBS

Synonyme

NSA1, WD repeat domain 74, WD repeat-containing protein 74

Filter:
  • Western Blot (WB) analysis of various lysates using WDR74 Recombinant monoclonal antibody (86361-2-RR)

    WB analysis using 86361-2-RR (same clone as 86361-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86361-2-RR (WDR74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86361-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WDR74 Recombinant monoclonal antibody (86361-2-RR)

    IF Staining of HeLa using 86361-2-RR (same clone as 86361-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR74 antibody (86361-2-RR, Clone: 251046A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86361-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86361-2-RR

    Affinity and Kinetic Characterization of 86361-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86361-2-RR against Human WDR74 were performed. The affinity constant is 1.02 nM.

Geprüfte Anwendungen

Produktinformation

86361-2-PBS bindet in WB, IF/ICC, Indirect ELISA WDR74 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen WDR74 fusion protein Ag14617
Vollständiger Name WD repeat domain 74
Berechnetes Molekulargewicht 385 aa, 42 kDa
Beobachtetes Molekulargewicht40 kDa
GenBank-ZugangsnummerBC006351
Gene symbol WDR74
Gene ID (NCBI) 54663
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR74, also known as WD repeat domain 74, is a protein-coding gene that plays important roles in various biological processes. WDR74 is involved in rRNA processing and ribosomal large subunit biogenesis. It is a 60S ribosome assembly factor, which is irreplaceable in ribosome biogenesis and is closely related to protein synthesis. WDR74 can induce nuclear β-catenin accumulation and activate Wnt-responsive genes, thus promoting cell proliferation and metastasis in various cancers.