WDR82 Polyklonaler Antikörper

WDR82 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 21354-1-AP

Synonyme

MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WD repeat domain 82, WDR82, WDR82A

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  WB analysis of HL-60 using 21354-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21354-1-AP (WDR82 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 21354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21354-1-AP (WDR82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21354-1-AP (WDR82 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21354-1-AP (WDR82 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat cells; K-562 cells
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

21354-1-AP bindet in WB, IHC, ELISA WDR82 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR82 fusion protein Ag15762
• Vollständiger Name: WD repeat domain 82
• Berechnetes Molekulargewicht: 313 aa, 35 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC018941
• Gene symbol: WDR82
• Gene ID (NCBI): 80335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Adv; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency; Authors - Chikako Okubo
human	WB	J Exp Clin Cancer Res; Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer; Authors - Macarena Morillo-Huesca

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mia (Verified Customer) (07-01-2025)

This antibody was incredibly helpful for my summer project while in medical school. It was used at a 1:100 dilution for staining BHPrS1 cells, and we are planning to drop the dilution to 1:500. It was also used at a 1:200 dilution for IHC of mouse and human prostate tissue, and worked wonderfully.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:200 for IHC, 1:100 for ICC
• Cell Tissue Type: Mouse & human prostate, BHPrS1 cells