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WEE1 Polyklonaler Antikörper

WEE1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29474-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using WEE1 Polyclonal antibody (29474-1-AP)

    WB analysis using 29474-1-AP (same clone as 29474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29474-1-AP (WEE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29474-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29474-1-PBS bindet in WB, Indirect ELISA WEE1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen WEE1 fusion protein Ag30194
Vollständiger Name WEE1 homolog (S. pombe)
Berechnetes Molekulargewicht 72 kDa
Beobachtetes Molekulargewicht72 kDa,110 kDa
GenBank-ZugangsnummerBC051831
Gene symbol WEE1
Gene ID (NCBI) 7465
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).