HE4 Polyklonaler Antikörper

HE4 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 14406-1-AP

Synonyme

WFDC2, WAP5, WAP four-disulfide core domain protein 2, WAP four disulfide core domain protein 2, Major epididymis-specific protein E4

---

• 

  IHC staining of human ovary tumor using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14406-1-AP (HE4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14406-1-AP (HE4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14406-1-AP (HE4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14406-1-AP (HE4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14406-1-AP (HE4 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human testis using 14406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14406-1-AP (HE4 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of MCF-7 using 14406-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 14406-1-AP (HE4 antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

14406-1-AP bindet in WB, IHC, IF/ICC, ELISA HE4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HE4 fusion protein Ag5684
• Vollständiger Name: WAP four-disulfide core domain 2
• Berechnetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC046106
• Gene symbol: HE4
• Gene ID (NCBI): 10406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

Publikationen

Species	Application	Title
		Biosens Bioelectron; An ultrasensitive ratiometric electrochemiluminescence immunosensor combining photothermal amplification for ovarian cancer marker detection.; Authors - Dandan Fang
mouse	WB	Oncotarget; Bcl-3 is a novel biomarker of renal fibrosis in chronic kidney disease.; Authors - Ran Chen
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang
human		RSC Adv; Machine learning algorithms enhance the specificity of cancer biomarker detection using SERS-based immunoassays in microfluidic chips; Authors - Nariman Banaei
human	IHC	Iran J Pathol; HE4, A New Potential Tumor Marker for Early Diagnosis and Predicting of Breast Cancer Progression.; Authors - Nazanin Mirmohseni Namini
human	IHC	Cell Rep; YAP targetome reveals activation of SPEM in gastric pre-neoplastic progression and regeneration; Authors - Adrian K H Loe