WFS1 Polyklonaler Antikörper

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• KD/KO Validated

WFS1 Polyklonal Antikörper für IHC, IF/ICC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Rezensionen (1)

Kat-Nr. : 26995-1-AP

Synonyme

WFRS, WFS, WOLFRAMIN

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  IHC staining of rat brain using 26995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26995-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:400 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 26995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 26995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 26995-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 26995-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 26995-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 26995-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 26995-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of rat brain using 26995-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HepG2 using 26995-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 13 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

26995-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ChIP, ELISA WFS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WFS1 fusion protein Ag25724
• Vollständiger Name: Wolfram syndrome 1 (wolframin)
• Berechnetes Molekulargewicht: 890 aa, 100 kDa
• GenBank-Zugangsnummer: BC030130
• Gene symbol: WFS1
• Gene ID (NCBI): 7466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss. Wolframin assembles into higher molecular weight complexes of approximately 400 kDa in the membrane(PMID: 12913071)

Publikationen

Species	Application	Title
human	IF	Sci Transl Med; Wolframin-1-expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice.; Authors - Jean-Christophe Delpech
mouse	IF	Neuron; Astrocyte-mediated regulation of BLAWFS1 neurons alleviates risk-assessment deficits in DISC1-N mice; Authors - Xinyi Zhou
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
rat,human	WB,IF	Nat Commun; WFS1 functions in ER export of vesicular cargo proteins in pancreatic β-cells.; Authors - Linlin Wang ; KO Validated
rat	IHC	Prog Neurobiol; The fasciola cinereum subregion of the hippocampus is important for the acquisition of visual contextual memory.; Authors - Seong-Beom Park
mouse	IF	Cell Rep; Acetylcholine synergizes with netrin-1 to drive persistent firing in the entorhinal cortex; Authors - Stephen D Glasgow

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jordan (Verified Customer) (01-25-2021)

Used free floating staining 16 hours in primary and 6 hours in secondary (Alexa 488+) at 4 degrees C. Antibody does a perfect job of labelling MEC layer 2 pyramidal neurons, alongside Parasubiculum neurons, some neurons in the subiculum and the majority of CA1 pyramidal neurons as widely reported in the literature

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse Brain/Pyramidal Neurons