WFS1 Polyklonaler Antikörper

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• KD/KO Validated

WFS1 Polyklonal Antikörper für IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26995-1-PBS

Synonyme

WFRS, WFS, WOLFRAMIN

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  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:400 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26995-1-AP (WFS1 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 26995-1-AP (same clone as 26995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WFS1 antibody (26995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26995-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26995-1-PBS bindet in IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA WFS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WFS1 fusion protein Ag25724
• Vollständiger Name: Wolfram syndrome 1 (wolframin)
• Berechnetes Molekulargewicht: 890 aa, 100 kDa
• GenBank-Zugangsnummer: BC030130
• Gene symbol: WFS1
• Gene ID (NCBI): 7466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss. Wolframin assembles into higher molecular weight complexes of approximately 400 kDa in the membrane(PMID: 12913071)