NSD3 Rekombinanter Antikörper

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• KD/KO Validated

NSD3 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230499C2

Kat-Nr. : 83116-2-PBS

Synonyme

WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3

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  WB analysis of A549 using 83116-2-RR (same clone as 83116-2-PBS)

  WB result of NSD3 antibody (83116-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSD3 transfected A549 cells. This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

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  WB analysis using 83116-2-RR (same clone as 83116-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83116-2-RR (WHSC1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00189-4

  Sandwich ELISA standard curve of MP00189-4, Human NSD3 Recombinant Matched Antibody Pair - PBS only. 83116-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1894. 83116-5-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

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  Sample test of MP00189-4

  The mean NSD3 concentration was determined to be 542.5 pg/mL in A549 cell extract based on a 1.0 mg/mL extract load, 565.1 pg/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 529.6 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  IHC staining of human lung squamous cell carcinoma using 83116-2-RR (same clone as 83116-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-2-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83116-2-RR (same clone as 83116-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSD3 antibody (83116-2-RR, Clone: 230499C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83116-2-RR (same clone as 83116-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug NSD3 Recombinant antibody (83116-2-RR, Clone:230499C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83116-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83116-2-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83116-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test NSD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NSD3 fusion protein Ag1894
• Vollständiger Name: Wolf-Hirschhorn syndrome candidate 1-like 1
• Berechnetes Molekulargewicht: 1437 aa, 162 kDa
• Beobachtetes Molekulargewicht: 160-170 kDa, 68-72 kDa
• GenBank-Zugangsnummer: BC012059
• Gene symbol: WHSC1L1
• Gene ID (NCBI): 54904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).