WISP-1/CCN4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WISP-1/CCN4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 18166-1-AP

Synonyme

WISP1, CCN4, WISP-1, Wnt-1-induced secreted protein, WNT1-inducible-signaling pathway protein 1

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  WB analysis using 18166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18166-1-AP (WISP-1/CCN4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18166-1-AP (WISP-1/CCN4 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, Mauslebergewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Lungenkarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18166-1-AP bindet in WB, IHC, IF, ELISA WISP-1/CCN4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WISP-1/CCN4 fusion protein Ag12657
• Vollständiger Name: WNT1 inducible signaling pathway protein 1
• Berechnetes Molekulargewicht: 367 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC074841
• Gene symbol: WISP1
• Gene ID (NCBI): 8840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WISP1( Wnt-induced secreted protein 1), also known as CCN4, is a secreted, matricellular protein allocated to the CCN protein family. The CCN protein family consists of six, modular structured, secreted proteins ( Cyr61, CTGF, Nov, WISP1, WISP2, WISP3 ). WISP1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. WISP1 is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. Its expression is associated with proliferation, cytoprotection, as well as extracellular matrix production. 18166-1-AP antibody detects the 40-45 kDa protein in SDS-PAGE.

Publikationen

Species	Application	Title
	WB	Am J Respir Crit Care Med; Critical Genomic Networks and Vasoreactive Variants in Idiopathic Pulmonary Arterial Hypertension.; Authors - Anna R Hemnes
human	WB,IF	Exp Mol Med; WISP-1 induced by mechanical stress contributes to fibrosis and hypertrophy of the ligamentum flavum through Hedgehog-Gli1 signaling.; Authors - Chao Sun ; KD Validated
human	WB	Food Chem Toxicol; Quinocetone induces mitochondrial apoptosis in HepG2 cells through ROS-dependent promotion of VDAC1 oligomerization and suppression of Wnt1/β-catenin signaling pathway.; Authors - Xiayun Yang
human	WB,IHC	Am J Transl Res; High expression of WISP-1 correlates with poor prognosis in pancreatic ductal adenocarcinoma.; Authors - Jian-Yu Yang
		Int J Oncol; Identification of WISP1 as a novel oncogene in glioblastoma.; Authors - Di Jing
human	WB	Oncol Lett; MMP12 is a potential therapeutic target for Adamantinomatous craniopharyngioma: Conclusions from bioinformatics analysis and in vitro experiments.; Authors - Minde Li