WNK3 Polyklonaler Antikörper

WNK3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32231-1-AP

Synonyme

Serine/threonine-protein kinase WNK3, Protein kinase with no lysine 3, Protein kinase lysine-deficient 3, PRKWNK3, KIAA1566

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  WB analysis using 32231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32231-1-AP (WNK3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32231-1-AP bindet in WB, ELISA WNK3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNK3 fusion protein Ag37713
• Vollständiger Name: WNK lysine deficient protein kinase 3
• Berechnetes Molekulargewicht: 1800aa, 198 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: NM_001002838
• Gene symbol: WNK3
• Gene ID (NCBI): 65267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNK3, is a member of the WNK (With No Lysine [K]) kinase family. It is a serine/threonine kinase that plays important roles in various physiological processes. WNK3 is expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain-specific. Isoform 3 is kidney-specific. Endogenous WNK3 protein is an active protein kinase when immunoprecipitated from cells and its overexpression increases the survival of HeLa cells by delaying the onset of apoptosis (PMID:16501604).