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WNK4 Rekombinanter Antikörper

WNK4 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250156A10

Kat-Nr. : 85886-4-PBS

Synonyme

EC:2.7.11.1, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Serine/threonine-protein kinase WNK4

Filter:
  • Western Blot (WB) analysis of HepG2 cells using WNK4 Recombinant antibody (85886-4-RR)

    WB analysis of HepG2 using 85886-4-RR (same clone as 85886-4-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85886-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WNK4 Recombinant antibody (85886-4-RR)

    WB analysis using 85886-4-RR (same clone as 85886-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85886-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85886-4-RR

    Affinity and Kinetic Characterization of 85886-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85886-4-RR against Human WNK4 were performed. The affinity constant is 0.926 nM.

Geprüfte Anwendungen

Produktinformation

85886-4-PBS bindet in WB, Indirect ELISA WNK4 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen WNK4 fusion protein Ag17773
Vollständiger Name WNK lysine deficient protein kinase 4
Berechnetes Molekulargewicht 1243 aa, 135 kDa
Beobachtetes Molekulargewicht 220 kDa
GenBank-ZugangsnummerBC136664
Gene symbol WNK4
Gene ID (NCBI) 65266
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNK4 (With No Lysine Kinase 4) is a member of the WNK family of serine/threonine protein kinases. It plays a crucial role in regulating electrolyte balance and blood pressure by modulating the activity of ion transporters in the kidney. WNK4 is primarily involved in the regulation of sodium and potassium homeostasis. It achieves this by phosphorylating and activating the kinases SPAK and OSR1, which in turn regulate the activity of the sodium-chloride cotransporter (NCC) in the distal convoluted tubule of the kidney. Additionally, by promoting sodium reabsorption and potassium secretion, WNK4 contributes to the regulation of blood pressure. Increased activity of WNK4 can lead to hypertension. (PMID: 24655003; 33491560)