WNT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WNT1 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(52)

Kat-Nr. : 27935-1-AP

Synonyme

Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, Proto oncogene Wnt 1, Proto oncogene Int 1 homolog, INT1

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  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 27935-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 27935-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT1 antibody (27935-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of NIH/3T3 using 27935-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  FC experiment of HeLa using 27935-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human WNT1 (27935-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 3T3-L1-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 52 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Produktinformation

27935-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA WNT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNT1 fusion protein Ag27380
• Vollständiger Name: wingless-type MMTV integration site family, member 1
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC074799
• Gene symbol: WNT1
• Gene ID (NCBI): 7471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Bioengineered; MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1); Authors - Guang-Lin Song
human	WB	Neural Regen Res; Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke; Authors - Jianfeng Wang
human	WB	Aging (Albany NY); Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.; Authors - Liansheng Sha
mouse	WB	J Ethnopharmacol; Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice; Authors - Yin OuYang
mouse	WB	Microb Biotechnol; Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice.; Authors - Xiaoxiao Zhao
mouse	WB	Mol Neurobiol; GPR30 Inhibits Neuronal Apoptosis After Subarachnoid Hemorrhage by Activating the Wnt/β-Catenin Pathway in a m6A-dependent Manner; Authors - Jun Peng