WNT10A Polyklonaler Antikörper

WNT10A Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 26238-1-AP

Synonyme

WNT10, Protein Wnt-10a, Protein Wnt 10a

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  IHC staining of human oesophagus cancer using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human oesophagus cancer using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human oesophagus cancer using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus cancer using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of NIH/3T3 using 26238-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 26238-1-AP (WNT10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 26238-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26238-1-AP (WNT10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

26238-1-AP bindet in WB, IHC, IF/ICC, ELISA WNT10A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNT10A fusion protein Ag23963
• Vollständiger Name: wingless-type MMTV integration site family, member 10A
• Berechnetes Molekulargewicht: 417 aa, 46 kDa
• GenBank-Zugangsnummer: BC052234
• Gene symbol: WNT10A
• Gene ID (NCBI): 80326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNT10A is a member of the WNT gene family, which consists of structurally related genes encoding secreted signaling molecules critical for many organogenesis processes. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines.

Publikationen

Species	Application	Title
human	IHC	Cancer Cell Int; Identification of the prognostic value of a 2-gene signature of the WNT gene family in UCEC using bioinformatics and real-world data.; Authors - Yuexin Hu
human	IHC	Oral Dis; Long non-coding RNAs MALAT1 and NEAT1 in non-syndromic orofacial clefts.; Authors - Errui Wang
goat	WB,IF	J Anim Sci Biotechnol; Melatonin promotes the development of the secondary hair follicles by regulating circMPP5; Authors - Xiaogao Diao
mouse	IHC	Kidney Int; Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis; Authors - Dong Zhou
human,mouse	WB,IHC	Cancer Biother Radiopharm; Raddeanin A Inhibits Colorectal Cancer Growth and Ameliorates Oxaliplatin Resistance Through the WNT/β-Catenin Signaling Pathway; Authors - Junguo Chen ; KD Validated
		Mol Ther Nucleic Acids; Mechanical stimuli-induced CCL2 restores adult mouse cells to regenerate hair follicles; Authors - Wang Wu