WNT2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

WNT2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

3H7B10

Kat-Nr. : 66656-1-Ig

Synonyme

3H7B10, Int 1 like protein 1, Int 1 related protein, INT1L1, Int-1-like protein 1

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  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of RAW 264.7 using 66656-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66656-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 66656-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66656-1-Ig (WNT2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

66656-1-Ig bindet in WB, IF/ICC, ELISA WNT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WNT2 fusion protein Ag25185
• Vollständiger Name: wingless-type MMTV integration site family member 2
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC029854
• Gene symbol: WNT2
• Gene ID (NCBI): 7472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

Publikationen

Species	Application	Title
human	WB,IF	Sci Adv; Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer; Authors - Atish Mohanty ; KD Validated
human	IF	Cell Mol Life Sci; α-Klotho released from HK-2 cells inhibits osteogenic differentiation of renal interstitial fibroblasts by inactivating the Wnt-β-catenin pathway.; Authors - Zewu Zhu
mouse	WB	PeerJ; Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin; Authors - Juan Yin ; KD Validated
human	WB	J Surg Res; Expression Analysis of ACSL5 and Wnt2B in Human Congenital Pulmonary Airway Malformations.; Authors - Yuan Qu
rat	WB	Int J Endocrinol; Fufang Xueshuantong Improves Diabetic Cardiomyopathy by Regulating the Wnt/β-Catenin Pathway; Authors - Meizhong Peng
human	WB	Int J Oncol; FUT2 promotes the tumorigenicity and metastasis of colorectal cancer cells via the Wnt/β‑catenin pathway; Authors - Peng Liu