WNT2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

WNT2 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H7B10

Kat-Nr. : 66656-1-PBS

Synonyme

3H7B10, Int 1 like protein 1, Int 1 related protein, INT1L1, Int-1-like protein 1

---

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66656-1-Ig (same clone as 66656-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66656-1-Ig (same clone as 66656-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66656-1-Ig (same clone as 66656-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66656-1-Ig (WNT2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66656-1-PBS bindet in WB, IF/ICC, Indirect ELISA WNT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WNT2 fusion protein Ag25185
• Vollständiger Name: wingless-type MMTV integration site family member 2
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC029854
• Gene symbol: WNT2
• Gene ID (NCBI): 7472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.