WNT3 Polyklonaler Antikörper

WNT3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28156-1-PBS

Synonyme

INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3

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  WB analysis using 28156-1-AP (same clone as 28156-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28156-1-AP (WNT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28156-1-AP (same clone as 28156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 28156-1-AP (same clone as 28156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28156-1-PBS bindet in WB, IHC, Indirect ELISA WNT3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNT3 fusion protein Ag27965
• Vollständiger Name: wingless-type MMTV integration site family, member 3
• Berechnetes Molekulargewicht: 355 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC114219
• Gene symbol: WNT3
• Gene ID (NCBI): 7473
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNT3 is also named as INT4. WNT3 is required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PMID: 14872406). Wnt3 is up-regulated in cancer tissues, such as gastric cancer and lung cancer. Wnt3, as a member of Wnt family, has been proved to promote the stabilization of β-catenin to regulate the radiation sensitivity of cancer cells (PMID: 31851619).