WNT3A Polyklonaler Antikörper

WNT3A Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(44)

Kat-Nr. : 26744-1-AP

Synonyme

Protein Wnt 3a, Protein Wnt-3a

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  WB analysis of fetal human brain using 26744-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 26744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 43 publications below
• IF: See 1 publications below

Produktinformation

26744-1-AP bindet in WB, IF, ELISA WNT3A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNT3A fusion protein Ag25051
• Vollständiger Name: wingless-type MMTV integration site family, member 3A
• Berechnetes Molekulargewicht: 352 aa, 39 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC103921
• Gene symbol: WNT3A
• Gene ID (NCBI): 89780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders. WNT3A is a secreted glycoprotein that is involved in both canonical and non-canonical Wnt signaling pathways. The canonical Wnt pathway, also known as the β-catenin-dependent pathway, regulates gene expression by stabilizing β-catenin, which then translocates to the nucleus and interacts with transcription factors like TCF/LEF. It's shown to promote trophectoderm formation in embryonic stem cells. It's reported that the canonical Wnt/β-catenin signaling pathway also contributes to the carcinogenesis and progression of lung cancer cell lines. The non-canonical pathways, which are β-catenin-independent, are involved in cytoskeletal organization and cell polarity.

Publikationen

Species	Application	Title
mouse	WB	Food Funct; Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism; Authors - Zehua Liu
rat	WB	Aging (Albany NY); Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.; Authors - Xin Zhang
rat	WB	Arch Toxicol; Developmental exposure to nonylphenol induced rat axonal injury in vivo and in vitro.; Authors - Siyao Li
human	WB	Genes Nutr; Arjunolic acid inhibits Wnt3a-mediated macrophage M2 polarization to suppress osteosarcoma progression; Authors - Jun Li
human	WB	Apoptosis; SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.; Authors - Zuo-Long Wu
human	WB	Biomed Pharmacother; BAIAP2L2 promotes the progression of gastric cancer via AKT/mTOR and Wnt3a/β-catenin signaling pathways.; Authors - Jianing Liu