WNT4 Polyklonaler Antikörper

WNT4 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 14371-1-AP

Synonyme

WNT 4, UNQ426/PRO864, SERKAL, Protein Wnt-4, Protein Wnt 4

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 14371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14371-1-AP (WNT4 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14371-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 14371-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberzirrhosegewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

14371-1-AP bindet in WB, IHC, IF/ICC, ELISA WNT4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNT4 fusion protein Ag5741
• Vollständiger Name: wingless-type MMTV integration site family, member 4
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC057781
• Gene symbol: WNT4
• Gene ID (NCBI): 54361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNT4 belongs to the Wnt family. WNT4 is critical for embryonic organogenesis, but later plays a unique role among Wnt proteins in the development and maintenance of müllerian and reproductive tissues (PMID: 33963381). The mesenchymal stem cells can improve cardiac function after cardiac injury by regulating endothelial cells via the Wnt4/β-catenin signaling pathway (PMID: 20551912). WNT4 may be modified by glycosylation, leading to an increase in molecular weight.

Publikationen

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Prenatal Perfluorooctanoic Acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4/β-catenin signaling pathway in progeny; Authors - Hua Du
human	WB	Endocrinology; PMID: 31748790; Authors - Alison M Neff
rat		Int J Clin Exp Pathol; Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1.; Authors - Junyi Hua
mouse	IHC	Neurochem Res; Wnt Signaling is Altered by Spinal Cord Neuronal Dysfunction in Amyotrophic Lateral Sclerosis Transgenic Mice.; Authors - Yu Li L
human	IF	Cell Death Dis; The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.; Authors - Nanchao Hong
mouse	IHC	BMC Complement Med Ther; Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathway.; Authors - Bing Cao