WNT5A/B Polyklonaler Antikörper

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• KD/KO Validated

WNT5A/B Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55184-1-PBS

Synonyme

WNT5A, hWNT5A, Protein Wnt 5a, Protein Wnt-5a, WNT5A B

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  WB analysis using 55184-1-AP (same clone as 55184-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55184-1-AP (same clone as 55184-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 55184-1-AP (same clone as 55184-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 55184-1-AP (same clone as 55184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55184-1-AP (WNT5A/B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55184-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55184-1-PBS bindet in WB, IHC, Indirect ELISA WNT5A/B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: wingless-type MMTV integration site family, member 5A
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: NM_003392
• Gene symbol: WNT5A
• Gene ID (NCBI): 7474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides. This antibody can recognize WNT5A and WNT5B.