WNT6 Polyklonaler Antikörper

WNT6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 24201-1-AP

Synonyme

Protein Wnt 6, WNT6

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Galerie der Validierungsdaten

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  WB analysis of SGC-7901 using 24201-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 24201-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24201-1-AP (WNT6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24201-1-AP (WNT6 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

24201-1-AP bindet in WB, IHC, IF, ELISA WNT6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNT6 fusion protein Ag20889
• Vollständiger Name: wingless-type MMTV integration site family, member 6
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC004329
• Gene symbol: WNT6
• Gene ID (NCBI): 7475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNT6 belongs to the Wnt family. It is ligand for members of the frizzled family of seven transmembrane receptors. WNT6 is likely to signal over only few cell diameters. WNT6 may be together with CAV1 to promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway. And WNT6 is mainly expressed in brain, endometrium epithelium, gastric cancer cell lines and gastric cancer tissues (PMID: 22370641).

Publikationen

Species	Application	Title
human,chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin
human	WB	Front Oncol; YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma.; Authors - Yang Bai
human	WB	Am J Physiol Cell Physiol; Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.; Authors - Qin Ju He
human	WB,IHC,IF	Gene; WNT6 participates in the occurrence and development of ovarian cancer by upregulating/activating the typical Wnt pathway and Notch1 signaling pathway; Authors - Haijuan Bao ; KD Validated
human	WB,IHC	Oncol Lett; Correlation study of DNA methylation of WNT6 gene with osteosarcoma in children.; Authors - Li Li
human	WB	Clin Transl Med; LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A; Authors - Danlei Chen