WNT7A/B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WNT7A/B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 10605-1-AP

Synonyme

WNT7B, WNT7A, Protein Wnt 7a, Protein Wnt-7a

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  WB analysis using 10605-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10605-1-AP (WNT7A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10605-1-AP

  IP result of anti-WNT7A (IP:10605-1-AP, 4ug; Detection:10605-1-AP 1:4000) with mouse kidney tissue lysate 2280 ug.

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  IHC staining of mouse placenta using 10605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 10605-1-AP (WNT7A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 10605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 10605-1-AP (WNT7A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10605-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (10605-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Mauslebergewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maus-Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

10605-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA WNT7A/B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WNT7A/B fusion protein Ag0874
• Vollständiger Name: wingless-type MMTV integration site family, member 7A
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 30-39 kDa
• GenBank-Zugangsnummer: BC008811
• Gene symbol: WNT7A
• Gene ID (NCBI): 7476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt7a is normally expressed in several organs, including the lung, testis, lymph node, and brain. As an oncogenic autocrine glycoprotein, Wnt7a promotes tumor invasion and distant metastasis with cancer-associated fibroblasts. Wnt7b is required for endothelial cells derived from pluripotent stem cells to acquire blood-brain barrier properties and may play an important role in the development of myopia in humans. This antibody can both recognize WNT7A and WNT7B.

Publikationen

Species	Application	Title
human	WB, IHC	Front Oncol; The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway.; Authors - Chun Tang
mouse	IF	Development; Aberrant centrosome biogenesis disrupts nephron and collecting duct progenitor growth and fate resulting in fibrocystic kidney disease; Authors - Tao Cheng
human	WB,IHC	Oncol Rep; Wnt7a predicts poor prognosis, and contributes to growth and metastasis in tongue squamous cell carcinoma.; Authors - Bo Jia ; KD Validated
mouse	WB	Behav Brain Res; OGDHL ameliorates cognitive impairment and Alzheimer's disease-like pathology via activating Wnt/β-catenin signaling in Alzheimer's disease mice.; Authors - Li Yao
	WB	Oncol Rep; RNA-binding motif protein 5 negatively regulates the activity of Wnt/β-catenin signaling in cigarette smoke-induced alveolar epithelial injury.; Authors - Yu-Qiu Hao
mouse	WB,IHC	Neurol Res; Wnt signaling pathway is involved in the pathogenesis of amyotrophic lateral sclerosis in adult transgenic mice.; Authors - Chen Yanchun Y