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WNT7A Polyklonaler Antikörper

WNT7A Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27177-1-PBS

Synonyme

Protein Wnt-7a, Protein Wnt 7a

Filter:
  • Western Blot (WB) analysis of various lysates using WNT7A Polyclonal antibody (27177-1-AP)

    WB analysis using 27177-1-AP (same clone as 27177-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27177-1-AP (WNT7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using WNT7A Polyclonal antibody (27177-1-AP)

    IP experiment of mouse kidney using 27177-1-AP (same clone as 27177-1-PBS)

    IP result of anti-WNT7A (IP:27177-1-AP, 4ug; Detection:27177-1-AP 1:2000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WNT7A Polyclonal antibody (27177-1-AP)

    IF Staining of HepG2 using 27177-1-AP (same clone as 27177-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (27177-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27177-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA WNT7A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen WNT7A fusion protein Ag26060
Vollständiger Name wingless-type MMTV integration site family, member 7A
Berechnetes Molekulargewicht 39 kDa
Beobachtetes Molekulargewicht 30-39 kDa
GenBank-ZugangsnummerBC008811
Gene symbol WNT7A
Gene ID (NCBI) 7476
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WNT7A belongs to the Wnt family. WNT7A plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PMID: 16826533). WNT7A is required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PMID:30026314). WNT7A is highly expressed in basal cells and is secreted in abundance by basal cells (PMID: 36318668).