WRAP53 Polyklonaler Antikörper

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• KD/KO Validated

WRAP53 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 14761-1-AP

Synonyme

WDR79, TCAB1, WD repeat-containing protein 79, WD40 repeat-containing protein antisense to TP53 gene, WRAP53beta

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  WB analysis using 14761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14761-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14761-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14761-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14761-1-AP

  IP result of anti-WRAP53 (IP:14761-1-AP, 4ug; Detection:14761-1-AP 1:300) with HeLa cells lysate 2480ug.

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  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14761-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, humanes Nierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

14761-1-AP bindet in WB, IHC, IF, IP, ELISA WRAP53 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WRAP53 fusion protein Ag6522
• Vollständiger Name: WD repeat containing, antisense to TP53
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 72-75 kDa
• GenBank-Zugangsnummer: BC002336
• Gene symbol: WRAP53
• Gene ID (NCBI): 55135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WRAP53 (WD40-encoding RNA Antisense to p53), also known as TCAB1 and WDR79, is a multifunctional protein encoded by a gene that produces both a coding mRNA and a regulatory antisense RNA targeting the tumor suppressor p53. Initially identified for its role in maintaining genomic stability by regulating p53 expression, WRAP53 is crucial for telomerase assembly and localization to Cajal bodies, impacting telomere maintenance. It also participates in DNA damage repair and cellular stress responses. The calculated molecular weight of WRAP53 is about 59 kDa, but the actual observed molecular weight is about 72-75 kDa, which may be due to modification.

Publikationen

Species	Application	Title
human	IF	Genes Dev; The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.; Authors - Sofia Henriksson
human	WB	Nat Chem Biol; Observation of processive telomerase catalysis using high-resolution optical tweezers.; Authors - Eric M Patrick
human	WB	Oncogenesis; RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.; Authors - Qunsong Tan ; KD Validated
	WB	Aging (Albany NY); Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism.; Authors - Xing-Hua Pan
human	WB,IF	RNA Biol; Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.; Authors - Christos Coucoravas ; KD Validated
human	WB	Cancer Cell Int; Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells.; Authors - Jing Niu