WRN Polyklonaler Antikörper

WRN Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25269-1-AP

Synonyme

DNA helicase, RecQ like type 3, Exonuclease WRN, RecQ protein like 2, RECQ3, RECQL2, RECQL3, WRN

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  WB analysis of MCF-7 using 25269-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25269-1-AP (WRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25269-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25269-1-AP (WRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25269-1-AP bindet in WB, ELISA WRN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Werner syndrome, RecQ helicase-like
• Berechnetes Molekulargewicht: 162 kDa
• Beobachtetes Molekulargewicht: 162 kDa
• GenBank-Zugangsnummer: NM_000553
• Gene symbol: WRN
• Gene ID (NCBI): 7486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Werner syndrome ATP dependent helicase (WRN) ,also named as RECQ3 or RecQ protein like 2, is a 1432 amino acid protein, which contains one HRDC domain, one helicase C-terminal domain, one helicase ATP-binding domain and 3'-5' exonuclease domain. WRN localizes in the nucleoplasm and belongs to the helicase family. WRN plays a central role in the repair of methylation-induced DNA damage and has an helicase activity necessary to prevent dramatic telomere loss during DNA replication. WRN gene mutation has an association with Werner syndrome and colorectal cancer.