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WSB1 Polyklonaler Antikörper

WSB1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11666-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using WSB1 Polyclonal antibody (11666-1-AP)

    WB analysis using 11666-1-AP (same clone as 11666-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11666-1-AP (WSB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WSB1 Polyclonal antibody (11666-1-AP)

    IHC staining of human prostate cancer using 11666-1-AP (same clone as 11666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11666-1-AP (WSB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WSB1 Polyclonal antibody (11666-1-AP)

    IF Staining of HepG2 using 11666-1-AP (same clone as 11666-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WSB1 antibody (11666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11666-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA WSB1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen WSB1 fusion protein Ag2211
Vollständiger Name WD repeat and SOCS box-containing 1
Berechnetes Molekulargewicht 421 aa, 47 kDa
Beobachtetes Molekulargewicht 56 kDa
GenBank-ZugangsnummerBC021110
Gene symbol WSB1
Gene ID (NCBI) 26118
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WSB1 (or SWIP1) gene encodes WD repeat and SOCS box-containing protein 1, a member of the WD-protein subfamily. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding 3 distinct isoforms have been found for this gene. WSB1 serves as a probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WSB1 has been reported (PMID: 15965468), initiated by the interaction of the two components (PMID: 18093972). WSB1 is also a part of an E3 ubiquitin ligase for the thyroid-hormone-activating type 2 iodothyronine deiodinase (D2) (PMID: 15965468).