WSB2 Polyklonaler Antikörper

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• KD/KO Validated

WSB2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12124-2-PBS

Synonyme

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  WB analysis of PC-3 using 12124-2-AP (same clone as 12124-2-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12124-2-AP (same clone as 12124-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12124-2-AP (same clone as 12124-2-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 12124-2-AP (same clone as 12124-2-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 12124-2-AP (same clone as 12124-2-PBS)

  IP result of anti-WSB2 (IP:12124-2-AP, 3ug; Detection:12124-2-AP 1:800) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 12124-2-AP (same clone as 12124-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using WSB2 antibody (12124-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12124-2-PBS bindet in WB, IF/ICC, IP, Indirect ELISA WSB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WSB2 fusion protein Ag2768
• Vollständiger Name: WD repeat and SOCS box-containing 2
• Berechnetes Molekulargewicht: 404 aa, 45 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC015887
• Gene symbol: WSB2
• Gene ID (NCBI): 55884
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WSB2, also named SBA2, encodes WD repeat and SOCS box-containing protein 2. WSB2 may be a substrate-recognition component of an E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. WSB2 is a novel protein supposed to be involved in male- and female-specific gonadal development, and also a regulator of IL-21 receptor expression and IL21-induced signal transduction. WSB2 might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological functions need further studies.