WT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WT1 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12609-1-PBS

Synonyme

Wilms tumor 1, Wilms tumor protein

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  WB analysis of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12609-1-AP (same clone as 12609-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 12609-1-AP (same clone as 12609-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 12609-1-AP (same clone as 12609-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 12609-1-AP (same clone as 12609-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12609-1-AP (same clone as 12609-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

  IP result of anti-WT1 (IP:12609-1-AP, 4ug; Detection:12609-1-AP 1:1000) with K-562 cells lysate 1360 ug. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

• 

  IF Staining of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using WT1 antibody (12609-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human WT1 (12609-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12609-1-PBS bindet in WB, IF/ICC, FC (Intra), IP, ELISA WT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WT1 fusion protein Ag3337
• Vollständiger Name: Wilms tumor 1
• Berechnetes Molekulargewicht: 449 aa, 49 kDa, 57 kDa
• Beobachtetes Molekulargewicht: 52-55 kDa
• GenBank-Zugangsnummer: BC032861
• Gene symbol: WT1
• Gene ID (NCBI): 7490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.