WT1 Rekombinanter Antikörper

WT1 Rekombinant Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2J13

Kat-Nr. : 82525-1-PBS

Synonyme

2J13, Wilms tumor 1, Wilms tumor protein

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  WB analysis using 82525-1-RR (same clone as 82525-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82525-1-RR (WT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 82525-1-RR (same clone as 82525-1-PBS)

  IP result of anti-WT1 (IP:82525-1-RR, 4ug; Detection:82525-1-RR 1:1000) with K-562 cells lysate 1600 ug. This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82525-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82525-1-RR against Human WT1 were performed. The affinity constant is 0.912 nM.

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Geprüfte Anwendungen

Produktinformation

82525-1-PBS bindet in WB, IP, Indirect ELISA WT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: WT1 fusion protein Ag19541
• Vollständiger Name: Wilms tumor 1
• Berechnetes Molekulargewicht: 449 aa, 49 kDa, 57 kDa
• Beobachtetes Molekulargewicht: 52-55 kDa
• GenBank-Zugangsnummer: BC032861
• Gene symbol: WT1
• Gene ID (NCBI): 7490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for the normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. The function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has a lower affinity for DNA and can bind RNA. WT1 exists some isoforms with a range of molecular weight is 33-50 kDa.