WTAP Monoklonaler Antikörper

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• KD/KO Validated

WTAP Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4A10G9

Kat-Nr. : 60188-1-PBS

Synonyme

4A10G9, Female-lethal(2)D homolog (hFL(2)D ), FL2D, KIAA0105, Mum2

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  WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

  IP result of anti-WTAP (IP:60188-1-Ig, 4ug; Detection:10200-1-AP 1:8000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 60188-1-Ig(WTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60188-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA WTAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WTAP fusion protein Ag0259
• Vollständiger Name: Wilms tumor 1 associated protein
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC000383
• Gene symbol: WTAP
• Gene ID (NCBI): 9589
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.