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WTAP Rekombinanter Antikörper

WTAP Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250657D4

Kat-Nr. : 81987-4-PBS

Synonyme

Female-lethal(2)D homolog (hFL(2)D ), FL2D, KIAA0105, Mum2, Pre-mRNA-splicing regulator WTAP

Filter:
  • Western Blot (WB) analysis of various lysates using WTAP Recombinant antibody (81987-4-RR)

    WB analysis using 81987-4-RR (same clone as 81987-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81987-4-RR (WTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81987-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using WTAP Recombinant antibody (81987-4-RR)

    IF Staining of Jurkat using 81987-4-RR (same clone as 81987-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using WTAP antibody (81987-4-RR, Clone: 250657D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81987-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81987-4-RR

    Affinity and Kinetic Characterization of 81987-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81987-4-RR against Human WTAP were performed. The affinity constant is 5.11 nM.

Geprüfte Anwendungen

Produktinformation

81987-4-PBS bindet in WB, IF/ICC, Indirect ELISA WTAP und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen WTAP fusion protein Ag0259
Vollständiger Name Wilms tumor 1 associated protein
Berechnetes Molekulargewicht 45 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC000383
Gene symbol WTAP
Gene ID (NCBI) 9589
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.