WWC2 Polyklonaler Antikörper

WWC2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 24750-1-AP

Synonyme

BH 3 only member B, BOMB, Protein WWC2, WW and C2 domain containing 2, WW domain containing protein 2, WWC2

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  WB analysis using 24750-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24750-1-AP (WWC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24750-1-AP

  IP result of anti-WWC2 (IP:24750-1-AP, 4ug; Detection:24750-1-AP 1:500) with HEK-293 cells lysate 1360 ug.

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  IHC staining of human kidney using 24750-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24750-1-AP (WWC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24750-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24750-1-AP (WWC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24750-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24750-1-AP (WWC2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 24750-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24750-1-AP (WWC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HEK-293 using 24750-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24750-1-AP (WWC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

24750-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA WWC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WWC2 fusion protein Ag20479
• Vollständiger Name: WW and C2 domain containing 2
• Berechnetes Molekulargewicht: 1192 aa, 134 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC017957
• Gene symbol: WWC2
• Gene ID (NCBI): 80014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WWC2 alson named as BOMB or WW and C2 domain containing 2 is 1192 amino acid protein, which contains 1 C2 domain and 2 WW domains. WWC2 localizes in cytoplasm and belongs to the WWC family. , WWC2 exists as seven alternatively spliced isoforms and is encoded by a gene located on human chromosome 4q35.1. WWC2 may be expressed in liver, brain, prostate and kidney. The function of WWC2 is still non-known and need to be further studied.

Publikationen

Species	Application	Title
mouse	WB	Nucleic Acids Res; TEAD1 regulates cell proliferation through a pocket-independent transcription repression mechanism; Authors - Feng Li
human	WB	Cell Signal; An integrative pan-cancer analysis of WWC family genes and functional validation in lung cancer; Authors - Hongmei Huang