WWOX Polyklonaler Antikörper

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• KD/KO Validated

WWOX Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15299-1-PBS

Synonyme

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  WB analysis using 15299-1-AP (same clone as 15299-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 15299-1-AP (same clone as 15299-1-PBS)

  WB result of WWOX antibody (15299-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWOX transfected MCF-7 cells. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 15299-1-AP (same clone as 15299-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse skeletal muscle using 15299-1-AP (same clone as 15299-1-PBS)

  IP result of anti-WWOX (IP:15299-1-AP, 4ug; Detection:15299-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 15299-1-AP (same clone as 15299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15299-1-PBS bindet in WB, IHC, IP, Indirect ELISA WWOX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WWOX fusion protein Ag7555
• Vollständiger Name: WW domain containing oxidoreductase
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC003184
• Gene symbol: WWOX
• Gene ID (NCBI): 51741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.