WWP1 Polyklonaler Antikörper

WWP1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28689-1-PBS

Synonyme

AIP5, Atrophin-1-interacting protein 5, EC:2.3.2.26, HECT-type E3 ubiquitin transferase WWP1, hSDRP1

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  WB analysis of HepG2 using 28689-1-AP (same clone as 28689-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 28689-1-AP (same clone as 28689-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 28689-1-AP (same clone as 28689-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 28689-1-AP (same clone as 28689-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28689-1-AP (same clone as 28689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28689-1-AP (same clone as 28689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 28689-1-AP (same clone as 28689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28689-1-AP (same clone as 28689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28689-1-PBS bindet in WB, IHC, Indirect ELISA WWP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WWP1 fusion protein Ag30161
• Vollständiger Name: WW domain containing E3 ubiquitin protein ligase 1
• Berechnetes Molekulargewicht: 922 aa, 105 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa
• GenBank-Zugangsnummer: BC036065
• Gene symbol: WWP1
• Gene ID (NCBI): 11059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.