WWP1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

WWP1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2G8G11

Kat-Nr. : 67804-1-Ig

Synonyme

2G8G11, AIP5, Atrophin-1-interacting protein 5, EC:2.3.2.26, HECT-type E3 ubiquitin transferase WWP1

---

• 

  WB analysis using 67804-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of HaCaT using 67804-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HaCaT-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

67804-1-Ig bindet in WB, ELISA WWP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WWP1 fusion protein Ag30171
• Vollständiger Name: WW domain containing E3 ubiquitin protein ligase 1
• Berechnetes Molekulargewicht: 922 aa, 105 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa
• GenBank-Zugangsnummer: BC036065
• Gene symbol: WWP1
• Gene ID (NCBI): 11059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse,human	WB	Cancers (Basel); GluOC Induced SLC7A11 and SLC38A1 to Activate Redox Processes and Resist Ferroptosis in TNBC; Authors - Jiaojiao Xu ; KD Validated